dtx3l protein expression level Search Results


dtx3l protein expression level  (GraphPad Software Inc)
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Chi-miR-483 targets the 3′-untranslated region (UTR) of deltex E3 ubiquitin ligase 3L <t>(DTX3L).</t> ( A ) The predicted binding site of chi-miR-483 in the 3′UTR of DTX3L according to bioinformatics analysis. ( B ) Design of the luciferase reporter. WT, the wildtype sequence of DTX3L-3′UTR contains the chi-miR-483 binding site; Mut, the sequence of DTX3L-3′UTR with a mutation in the chi-miR-483 binding site. ( C ) 293T cells were co-transfected with wildtype (WT) or mutant (Mut) luciferase reports of DTX3L 3′UTR with chi-miR-483 mimics or negative control (NC) mimics. The luciferase reporter assay demonstrated that chi-miR-483 significantly decreased the luciferase activity of DTX3L WT in 293T cells. Data are shown as the mean ± SEM values ( n = 3, ** p < 0.01, Student’s t -test).
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Chi-miR-483 targets the 3′-untranslated region (UTR) of deltex E3 ubiquitin ligase 3L (DTX3L). ( A ) The predicted binding site of chi-miR-483 in the 3′UTR of DTX3L according to bioinformatics analysis. ( B ) Design of the luciferase reporter. WT, the wildtype sequence of DTX3L-3′UTR contains the chi-miR-483 binding site; Mut, the sequence of DTX3L-3′UTR with a mutation in the chi-miR-483 binding site. ( C ) 293T cells were co-transfected with wildtype (WT) or mutant (Mut) luciferase reports of DTX3L 3′UTR with chi-miR-483 mimics or negative control (NC) mimics. The luciferase reporter assay demonstrated that chi-miR-483 significantly decreased the luciferase activity of DTX3L WT in 293T cells. Data are shown as the mean ± SEM values ( n = 3, ** p < 0.01, Student’s t -test).

Journal: Biomolecules

Article Title: Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats

doi: 10.3390/biom11030472

Figure Lengend Snippet: Chi-miR-483 targets the 3′-untranslated region (UTR) of deltex E3 ubiquitin ligase 3L (DTX3L). ( A ) The predicted binding site of chi-miR-483 in the 3′UTR of DTX3L according to bioinformatics analysis. ( B ) Design of the luciferase reporter. WT, the wildtype sequence of DTX3L-3′UTR contains the chi-miR-483 binding site; Mut, the sequence of DTX3L-3′UTR with a mutation in the chi-miR-483 binding site. ( C ) 293T cells were co-transfected with wildtype (WT) or mutant (Mut) luciferase reports of DTX3L 3′UTR with chi-miR-483 mimics or negative control (NC) mimics. The luciferase reporter assay demonstrated that chi-miR-483 significantly decreased the luciferase activity of DTX3L WT in 293T cells. Data are shown as the mean ± SEM values ( n = 3, ** p < 0.01, Student’s t -test).

Article Snippet: Differences in wildtype (WT) or mutant (Mut) DTX3L 3′UTR luciferase reports and the expression level of DTX3L protein under two conditions were compared using Student’s t -test (GraphPad Prism version 8.0, San Diego, CA, USA).

Techniques: Ubiquitin Proteomics, Binding Assay, Luciferase, Sequencing, Mutagenesis, Transfection, Negative Control, Reporter Assay, Activity Assay

Immunohistochemical analysis of DTX3L in the C16 and P16 uterus. ( A ) Images stained with DTX3L antibodies. The positive signal of DTX3L was distinctly detected in the uterine luminal epithelium or glandular epithelium in P16. The section stained with nonrelevant immunoglobulin G served as the negative control (NC). ( B ) Quantitative analysis of DTX3L by measuring the average integrated optical density (IOD) in the endometrium. Asterisks indicate significant differences (mean ± SEM) between C16 and P16 (*** p < 0.001); the p -value was determined by Student’s t -test. Legend: LE, endometrial luminal epithelium; GE, glandular epithelium. Scale bar = 100 μm.

Journal: Biomolecules

Article Title: Differential MicroRNA Expression Involved in Endometrial Receptivity of Goats

doi: 10.3390/biom11030472

Figure Lengend Snippet: Immunohistochemical analysis of DTX3L in the C16 and P16 uterus. ( A ) Images stained with DTX3L antibodies. The positive signal of DTX3L was distinctly detected in the uterine luminal epithelium or glandular epithelium in P16. The section stained with nonrelevant immunoglobulin G served as the negative control (NC). ( B ) Quantitative analysis of DTX3L by measuring the average integrated optical density (IOD) in the endometrium. Asterisks indicate significant differences (mean ± SEM) between C16 and P16 (*** p < 0.001); the p -value was determined by Student’s t -test. Legend: LE, endometrial luminal epithelium; GE, glandular epithelium. Scale bar = 100 μm.

Article Snippet: Differences in wildtype (WT) or mutant (Mut) DTX3L 3′UTR luciferase reports and the expression level of DTX3L protein under two conditions were compared using Student’s t -test (GraphPad Prism version 8.0, San Diego, CA, USA).

Techniques: Immunohistochemical staining, Staining, Negative Control